David Gazzo1,
Jeremiah Zartman1
1. University of Notre Dame
01/17/2025
Ex vivo calcium imaging in Drosophila opens an expansive amount of research avenues for the study of live signal propagation through complex tissue. Here, we describe how to isolate Drosophila organs of interest, like the developing wing imaginal disc and larval brain, culture them for extended periods, up to 10 hours, and how to image the calcium dynamics occurring within them using genetically encoded biosensors like GCaMP.
Researchers should cite this dataset as follows:
Gazzo, D., Zartman, J. (2025). Calcium Imaging in Drosophila Organs. Purdue Unversity EMBRIO Data Inventory.
The dataset is licensed under Attribution 4.0 International (CC BY 4.0).